actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.12737/23401actabiomedica-287Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В БИОЛОГИИ И МЕДИЦИНЕEXPERIMENTAL RESEARCHES IN BIOLOGY AND MEDICINEРеконструкция сайтов рекомбинации в геномных структурах штаммов генотипа 6 вируса гепатита СReconstruction of recombination sites in genomic structures of the strains of genotype 6 of hepatitis C virusГаращенкоН. Е.GarashchenkoN. E.nadzelin@mail.ruДжиоевЮ. П.DzhioevY. P.alanir07@mail.ruПарамоновА. И.ParamonovA. I.paramonov_a.i@mail.ruСтепаненкоЛ. А.StepanenkoL. A.steplia@mail.ruМаловС. И.MalovS. I.noemail@neicon.ruКолбасееваО. В.KolbaseevaO. V.kolbaseeva@yandex.ruМаловИ. В.MalovI. V.noemail@neicon.ruЗлобинВ. И.ZlobinV. I.vizlobin@mail.ruФГБОУ ВО «Иркутский государственный медицинский университет» Минздрава РоссииIrkutsk State Medical UniversityФГБОУ ВО «Иркутский государственный медицинский университет» Минздрава России; ФГБНУ «Научный центр проблем здоровья семьи и репродукции человека»Irkutsk State Medical University; Scientific Centre for Family Health and Human Reproduction Problems20162809201615100103Copyright © Гаращенко Н.Е., Джиоев Ю.П., Парамонов А.И., Степаненко Л.А., Малов С.И., Колбасеева О.В., Малов И.В., Злобин В.И., 20162016Гаращенко Н.Е., Джиоев Ю.П., Парамонов А.И., Степаненко Л.А., Малов С.И., Колбасеева О.В., Малов И.В., Злобин В.И.Garashchenko N.E., Dzhioev Y.P., Paramonov A.I., Stepanenko L.A., Malov S.I., Kolbaseeva O.V., Malov I.V., Zlobin V.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/287

В геномах 46 штаммов генотипа 6 вируса гепатита С посредством комплекса биоинформационных программ RDP выявлены 6 штаммов-рекомбинантов, в которых зафиксированы 7 сайтов рекомбинации. Для них определены родительские штаммы, от которых они могли быть получены. Большая часть сайтов рекомбинации приходится на область структурных генов C, E1 и E2 и неструктурных генов NS5a и NS5b. В одном штамме выявлен уникальный сайт рекомбинации в высоко консервативном гене NS3. По результатам исследования определены «горячие точки» рекомбинации в штаммах генотипа 6 вируса гепатита С.

The encoded portion of the complete genomes of 46 strains of the genotype 6 of hepatitis C virus through bioinformatics RDP programs complex group of 6 recombinants strains was identified, in which 7 recombination sites were fixed. Strains correspond to the three-recombinant HCV subtypes: 6a, 6b and 61. For each of the identified recombinant we defined parent strains from which they can be obtained. Three recombinants were obtained from parent strains of the same subtype (homologous inside subgenotypic recombination). For the remaining three recombinants parent strains were members of three different subtypes (between subgenotypic recombination).In one strain we identified a unique recombination site in a highly conservative NS3 gene. Most of the recombination sites occurred in the region of the structural genes C, E1 and E2, and in the area of non-structural genes NS5a and NS5b.In the recombinant strain DQ480518-6a two recombination site were identified. One site is located in the structural and nonstructural genes (E2 + NS1 + NS2), and a second one in non-structural region. Dimensions of recombination sites can vary from 86 to 1072 nucleotide bases. The study identified "hot spots" of recombination in the strains of genotype 6 of hepatitis C virus. The recombinants were found in the population of the three countries: the United States (from the serum of an immigrant), Hong Kong and China.

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The authors declare that there are no conflicts of interest present.