actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.29413/ABS.2021-6.1.9actabiomedica-2617Research ArticleМИКРОБИОЛОГИЯ И ВИРУСОЛОГИЯMICROBIOLOGY AND VIRUSOLOGYЭпизоотическая ситуация по анаплазмозу мелких жвачных животных на территории Иркутской областиEpizootic Situation on Anaplasmosis of Small Ruminants in the Irkutsk Regionhttps://orcid.org/0000-0003-4057-2890СунцоваО. В.SuntsovaO. V.

Сунцова Ольга Владимировна – кандидат биологических наук, научный сотрудник лаборатории молекулярной эпидемиологии и генетической диагностики

664003, г. Иркутск, ул. Тимирязева 16

Olga V. Suntsova – Cand. Sc. (Biol.), Research Officer at the Laboratory of Molecular Epidemiology and Genetic Diagnostics

Timiryazeva str. 16, Irkutsk 664003

olga_syntsova@list.ruhttps://orcid.org/0000-0002-5930-5306РарВ. А.RarV. A.

Рар Вера Александровна – кандидат биологических наук, научный сотрудник

630090, г. Новосибирск, пр. Лаврентьева, 8

Vera A. Rar – Cand. Sc. (Biol.), Research Officer

Lavrentyeva av. 8, Novosibirsk 630090

rarv@niboch.nsc.ruhttps://orcid.org/0000-0003-3909-7551ЛисакО. В.LisakO. V.

Лисак Оксана Васильевна – младший научный сотрудник лаборатории молекулярной эпидемиологии и генетической диагностики

664003, г. Иркутск, ул. Тимирязева 16

Oksana V. Lisak – Junior Research Officer at the Laboratory of Molecular Epidemiology and Genetic Diagnostics

Timiryazeva str. 16, Irkutsk 664003

lisak.liza@rambler.ruhttps://orcid.org/0000-0001-8566-7004МельцовИ. В.MeltsovI. V.

Мельцов Иван Владимирович – кандидат ветеринарных наук, доцент

664038, Иркутская обл., Иркутский р-н, п. Молодёжный

Ivan V. Meltsov – Cand. Sc. (Vet.), Associate Professor

Molodezhny settlement, Irkutsk region 664038

ivanmeltsov@mail.ruhttps://orcid.org/0000-0002-8209-616XДорощенкоЕ. К.DoroschenkoE. K.

Дорощенко Елена Константиновна – кандидат биологических наук, научный сотрудник лаборатории молекулярной эпидемиологии и генетической диагностики

664003, г. Иркутск, ул. Тимирязева 16

Elena K. Doroshchenko – Cand. Sc. (Biol.), Research Officer at the Laboratory of Molecular Epidemiology and Genetic Diagnostics

Timiryazeva str. 16, Irkutsk 664003

doroshchenko-virus@mail.ruhttps://orcid.org/0000-0001-8183-1233СавиноваЮ. С.SavinovaYu. S.

Савинова Юлия Сергеевна – младший научный сотрудник лаборатории молекулярной эпидемиологии и генетической диагностики

664003, г. Иркутск, ул. Тимирязева 16

Yulia S. Savinova – Junior Research Officer at the Laboratory of Molecular Epidemiology and Genetic Diagnostics

Timiryazeva str. 16, Irkutsk 664003

vippersona2389@rambler.ruhttps://orcid.org/0000-0001-5613-5447ТикуновА. Ю.TikunovA. Yu.

Тикунов Артем Юрьевич – научный сотрудник 

630090, г. Новосибирск, пр. Лаврентьева, 8

Artyom Yu. Tikunov – Research Officer

Lavrentyeva av. 8, Novosibirsk 630090

arttik@ngs.ruhttps://orcid.org/0000-0002-6324-8746КозловаИ. В.KozlovaI. V.

Козлова Ирина Валерьевна – доктор медицинских наук, заведующая лабораторией молекулярной эпидемиологии и генетической диагностики

664003, г. Иркутск, ул. Тимирязева 16

Irina V. Kozlova – Dr. Sc. (Med.), Head of the Laboratory of Molecular Epidemiology and Genetic Diagnostics

Timiryazeva str. 16, Irkutsk 664003

iwerhoz@rambler.ruФГБНУ «Научный центр проблем здоровья семьи и репродукции человека»Scientific Centre for Family Health and Human Reproduction ProblemsФГБУН Институт химической биологии и фундаментальной медицины Сибирского отделения Российской академии наукInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of SciencesФГБОУ ВО Иркутский государственный аграрный университет им. А.А. ЕжевскогоIrkutsk State University of Agriculture named after A.A. Ezhevsky202109042021616068Copyright © Сунцова О.В., Рар В.А., Лисак О.В., Мельцов И.В., Дорощенко Е.К., Савинова Ю.С., Тикунов А.Ю., Козлова И.В., 20212021Сунцова О.В., Рар В.А., Лисак О.В., Мельцов И.В., Дорощенко Е.К., Савинова Ю.С., Тикунов А.Ю., Козлова И.В.Suntsova O.V., Rar V.A., Lisak O.V., Meltsov I.V., Doroschenko E.K., Savinova Y.S., Tikunov A.Y., Kozlova I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/2617

Анаплазмозы жвачных животных – это группа природно-очаговых инфекций, вызываемых бактериями из рода Anaplasma семейства Anaplasmataceae. Основным этиологическим агентом анаплазмоза овец, коз, а также диких жвачных животных является Anaplasma ovis, которая паразитирует в эритроцитах этих животных. На сегодняшний день информация о наличии очагов анаплазмоза мелких жвачных животных на территории Иркутской области отсутствовала. Цель данной работы – индикация и идентификация анаплазм в крови овец и коз с помощью молекулярно-генетических методов, получение данных о широте распространения очагов анаплазмозов на территории Иркутской области. На наличие ДНК анаплазм были исследованы 20 образцов крови коз, 611 образцов крови овец и 209 клещей Dermacentor nuttalli из 12 районов Иркутской области. Только один вид анаплазм – A. ovis – был обнаружен среди генотипированных образцов. A. ovis была обнаружена в крови овец и коз во всех исследуемых районах Иркутской области. Доля образцов крови овец, содержащих ДНК анаплазм, варьировала от 30 % до 85 %, коз – от 10 % до 100 % в разных районах региона, составив в среднем по Иркутской области у овец – 57,8 %, у коз – 55,0 %. Заражённость A. ovis клещей D. nuttalli составила 5,7 %. A. ovis выявлена у домашних животных, принадлежащих различным собственникам, что свидетельствует о широком распространении очагов анаплазмоза домашних мелких жвачных животных на территории региона. Нуклеотидные последовательности образцов, выявленные в крови овец и коз на территории Иркутской области, различались между собой единичной нуклеотидной заменой и были идентичны последовательностям типового штамма Haibei, а также последовательностям A. ovis, обнаруженным ранее в крови овец из Монголии, оленей из Китая, а также клещей Dermacentor niveus и Dermacentor nuttalli из Китая. Эти последовательности также были идентичны последовательностям, обнаруженным ранее в крови овец из Алтая и в клещах D. nuttalli из Тувы, что свидетельствует о широком распространении данных геновариантов A. ovis на территории Сибири и о вероятной роли клещей D. nuttalli в качестве переносчика возбудителя анаплазмоза мелких жвачных животных в Иркутской области.

Anaplasmosis of ruminants is a group of natural focal infections caused by bacteria from the genus Anaplasma of the Anaplasmataceae family. The main etiological agent of anaplasmosis in sheep, goats, and wild ruminants is Anaplasma ovis, which parasitizes in the erythrocytes of these animals. The purpose of this study was the finding and identification of Anaplasma spp. in the blood of small ruminants using genetic methods and obtaining data on the distribution of anaplasmosis in the Irkutsk region. 20 goat blood samples, 611 sheep blood samples and 209 Dermacentor nuttalli ticks from 12 districts of the Irkutsk region were examined for the presence of Anaplasma spp. Only one type of anaplasma, A. ovis, was found among the genotyped samples. A. ovis was found in the blood of sheep and goats in all of the studied districts of the Irkutsk region. The proportion of sheep blood samples containing anaplasma DNA varied from 30 % to 85 %, in goats – from 10 % to 100 % in different districts, and averaged 57.8 % in sheep and 55,0 % in goats. Frequency of infection of D. nuttalli ticks with A. ovis was 5.7 %. The nucleotide sequences of the samples detected in the blood of small ruminants on the territory of the Irkutsk region differed from each other by a single nucleotide substitution and were identical to the sequences of the type strain Haibei, as well as the sequences of A. ovis previously found in the blood of sheep from Mongolia, deer from China, and Dermacentor niveus and Dermacentor nuttalli ticks from China. These sequences were also identical to the sequences previously found in the blood of sheep from Altai and in Dermacentor nuttalli ticks from Tuva, which indicates the wide distribution of these A. ovis genovariants in Siberia and the probable role of D. nuttalli as a carrier of the agent of anaplasmosis of small ruminants in the Irkutsk region.

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The authors declare that there are no conflicts of interest present.