actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.12737/21612actabiomedica-228Research ArticleМИКРОБИОЛОГИЯ И ВИРУСОЛОГИЯMICROBIOLOGY AND VIRUSOLOGYМолекулярно-биологическая характеристика Yersinia pseudotuberculosis и Yersinia enterocolitica, выделенных в Сибири и на Дальнем ВостокеBiomolecular characteristics of Yersinia pseudotuberculosis and Yersinia enterocolitica isolated in Siberia and in the Far EastКаримоваТ. В.KarimovaT. V.adm@chumin.irkutsk.ruКлимовВ. Т.KlimovV. T.noemail@neicon.ruЧесноковаМ. В.ChesnokovaM. V.noemail@neicon.ruФКУЗ Иркутский научно-исследовательский противочумный институт Сибири и Дальнего Востока РоспотребнадзораCenter of Hygiene and Epidemiology in Novosibirsk RegionФБУЗ «Центр гигиены и эпидемиологии в Новосибирской области»Irkutsk Antiplague Research Institute of Siberia and Far East of Rospotrebnadzor20162805201613(1)6064Copyright © Каримова Т.В., Климов В.Т., Чеснокова М.В., 20162016Каримова Т.В., Климов В.Т., Чеснокова М.В.Karimova T.V., Klimov V.T., Chesnokova M.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/228

Доминирующий в регионе генотип Y pseudotuberculosis представлен 0:1b серотипом первой геногруппы (pYV+, ympA+, HPI), содержит плазмиды 47:82 МDа или только 47 МDа, отличается спектром риботипов и фингерпринтов. Y. enterocolitica 2-4 биотипов характеризуется наличием плазмиды pYV, генов ail, ystA, установлен уникальный только для России фаготип Xz (3/0:3). Подтверждена роль в качестве этиологического агента Y. enterocolitica 1A биотипа, содержащего ген термостабильного токсина ystB.

A special feature of Y. pseudotuberculosis strains is its biochemical uniformity irrespective of the time and location of the causative agent isolation and the existence of 21 serological variants. Y. enterocolitica is a quite a heterogeneous species and is classified into 6 biochemical types associated with 29 serological variants. 221 Y. pseudotuberculosis and 447 Y. enterocolitica strains in total isolated in Siberia and in the Far East were characterized. Y. pseudotuberculosis genotype dominating in the Siberian and Far Eastern regions is presented by 0:1b serotype of the first genogroup (pYV+, ympA+, HPI-) in two- (47:82 МDa) or single-plasmid (47 МDа) variants. Ribotyping and fingerprinting revealed 8 and 10 Y. pseudotuberculosis genotypes, respectively, that indicated relative heterogeneity of the circulated strains. Regional difference of ribotypes and fingerprints was noted. 401 of447 Y. enterocolitica strains were classified as biotype A1 including 11 serotypes (0:4,32; 0:4,44; 0:5; 0:6,30; 0:6,31; 0;7,8; 0:12,25; 0:13,7; O:19,8; 0:41,43) and 46 strains belonged to biotypes 2-4 of O:3 and O:9 serotypes. Y. enterocolitica strains of biotypes 1A were isolated both from the environments, animals and patient samples as like the representatives of biotypes 2-4. The differentiating tests of fucose and sorbose made it possible to identify two species new for the Russian Federation - Y. mollaretii and Y. bercovierii. Y. enterocolitica biotypes 2-4 carried pYV plasmid and chromosomal ail, ystA virulence genes. These strains were referred to phagotypes X3 (2/0:9) and VIII (4/0:3) and also to phagotype Xz (3/0:3), unique for Russia. Y. enterocolitica biotype 1A containing ystB thermostable toxin gene was confirmed to be an infectious etiological agent.

факторы патогенностигенотипирование штаммовYersinia pseudotuberculosisYersinia enterocoliticaYersinia pseudotuberculosisYersinia enterocoliticapathogenicity factorstrains genotypingReferencesЕвсеева В.В., Платонов М.Е., Дентовская С.В., Анисимов А.П. Типирование Yersinia pseudotuberculosis с помощью мультилокусного анализа вариабельного числа тандемных повторов // Проблемы особо опасных инфекций. - 2015. - Вып. 2. - С. 55-57Евсеева В.В., Платонов М.Е., Дентовская С.В., Анисимов А.П. Типирование Yersinia pseudotuberculosis с помощью мультилокусного анализа вариабельного числа тандемных повторов // Проблемы особо опасных инфекций. - 2015. - Вып. 2. - С. 55-57Шурыгина И.А., Чеснокова М.В., Климов В.Т., Малов И.В., Марамович А.С. Псевдотуберкулез. - Новосибирск: Наука, 2003. - 320 сШурыгина И.А., Чеснокова М.В., Климов В.Т., Малов И.В., Марамович А.С. Псевдотуберкулез. - Новосибирск: Наука, 2003. - 320 сBogdanovich T. (2003). Use of O-antigen gene cluster-specific PCRs for the identification and O-geno-typing of Yersinia pseudotuberculosis and Yersinia pestis. J. Clin. Microbiol., (41), 5103-5112.Bogdanovich T. (2003). Use of O-antigen gene cluster-specific PCRs for the identification and O-geno-typing of Yersinia pseudotuberculosis and Yersinia pestis. J. Clin. Microbiol., (41), 5103-5112.Carniel E. (1999). The Yersinia high-pathogenicity island. Int. Microbiol., 2 (3), 161-167.Carniel E. (1999). The Yersinia high-pathogenicity island. Int. Microbiol., 2 (3), 161-167.Fukushima H., Matsuda Y., Seki R., Tsubokura M., Takeda N., Shubin F.N., Paik I.K., Zheng X.B. (2001). Geographical heterogeneity between Far Eastern and Western countries in prevalence of the virulence plasmid, the superantigen Yersinia pseudotuberculosis-derived mitogen, and the high-pathogenicity island among Yersinia pseudotuberculosis strains. J. Clin. Microbiol., 39 (10), 3541-3547.Fukushima H., Matsuda Y., Seki R., Tsubokura M., Takeda N., Shubin F.N., Paik I.K., Zheng X.B. (2001). Geographical heterogeneity between Far Eastern and Western countries in prevalence of the virulence plasmid, the superantigen Yersinia pseudotuberculosis-derived mitogen, and the high-pathogenicity island among Yersinia pseudotuberculosis strains. J. Clin. Microbiol., 39 (10), 3541-3547.Ibrahim A., Liesack W., Stackebrandt E. (1992). Polymerase chain reaction-gene profe detection system specific for pathogenic strains of Yersinia enterocolitica. J. Clin. Microbiol, 30 (8), 1942-1947.Ibrahim A., Liesack W., Stackebrandt E. (1992). Polymerase chain reaction-gene profe detection system specific for pathogenic strains of Yersinia enterocolitica. J. Clin. Microbiol, 30 (8), 1942-1947.Kieres T. (1984). Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid., 12 (1), 19-36.Kieres T. (1984). Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid., 12 (1), 19-36.Sihvonen L.M., Jalkanen K., Huovinen E., Toivonen S., Corander J., Kuusi M., Skurnik M., Siitonen A., Haukka K. (2012). Clinical isolates of Yersinia enterocolitica biotype 1A represent two phylogenetic lineages with differing pathogenicity-related properties. BMC Microbiol., (12), 208-219.Sihvonen L.M., Jalkanen K., Huovinen E., Toivonen S., Corander J., Kuusi M., Skurnik M., Siitonen A., Haukka K. (2012). Clinical isolates of Yersinia enterocolitica biotype 1A represent two phylogenetic lineages with differing pathogenicity-related properties. BMC Microbiol., (12), 208-219.Thoerner P., Bin Kingombe C.I., Bögli-Stuber K., Bissig-Choisat B., Wassenaar T.M., Frey J., Jemmi T. (2003). PCR detection of virulence genes in Yesinia enterocolitica and Yesinia pseudotuberculosis and investigation of virulence gene distribution. Appl. Environ. Microbiol., 69 (3), 1810-1816.Thoerner P., Bin Kingombe C.I., Bögli-Stuber K., Bissig-Choisat B., Wassenaar T.M., Frey J., Jemmi T. (2003). PCR detection of virulence genes in Yesinia enterocolitica and Yesinia pseudotuberculosis and investigation of virulence gene distribution. Appl. Environ. Microbiol., 69 (3), 1810-1816.Uchiyama T., Miyoshi-Akiyama T., Kato H., Fujimaki W., Imanishi K., Yan X.J. (1993). Superantigen properties of a novel mitogenic substance produced by Yersinia pseudotuberculosis isolated from patients manifesting acute and systemic symptoms. J. Immunol., 151 (8), 4407-4413.Uchiyama T., Miyoshi-Akiyama T., Kato H., Fujimaki W., Imanishi K., Yan X.J. (1993). Superantigen properties of a novel mitogenic substance produced by Yersinia pseudotuberculosis isolated from patients manifesting acute and systemic symptoms. J. Immunol., 151 (8), 4407-4413.Voskressenskaya E., Leclercq A., Tseneva G., Carniel E. (2005). Evaluation of ribotyping as a tool for molecular typing of Yersinia pseudotuberculosis strains of worldwide origin. J. Clin. Microbiol., 43 (12), 6155-6160.Voskressenskaya E., Leclercq A., Tseneva G., Carniel E. (2005). Evaluation of ribotyping as a tool for molecular typing of Yersinia pseudotuberculosis strains of worldwide origin. J. Clin. Microbiol., 43 (12), 6155-6160.Voskresenskaya E., Savin C., Leclercq A., Tseneva G., Carniel E. (2014). Typing and clustering of Yersinia pseudotuberculosis isolates by restriction fragment length polymorphism analysis using insertion sequences. J. Clin. Microbiol., 52 (6), 1978-1989.Voskresenskaya E., Savin C., Leclercq A., Tseneva G., Carniel E. (2014). Typing and clustering of Yersinia pseudotuberculosis isolates by restriction fragment length polymorphism analysis using insertion sequences. J. Clin. Microbiol., 52 (6), 1978-1989.Wannet W.J., Reessink M., Brunings H., Maas H.M. (2001). Detection of pathogenic Yersinia enterocolitica a rapid and sensitive duplex PCR assay. J. Clin. Microbiol., 39 (12), 4483-4486.Wannet W.J., Reessink M., Brunings H., Maas H.M. (2001). Detection of pathogenic Yersinia enterocolitica a rapid and sensitive duplex PCR assay. J. Clin. Microbiol., 39 (12), 4483-4486.Wauters G., Kandolo K., Jansens M. (1987). Revised biogrouping scheme of Yesinia enterocolitica. Contrib. Microbiol. Immunol., (9), 14-21.Wauters G., Kandolo K., Jansens M. (1987). Revised biogrouping scheme of Yesinia enterocolitica. Contrib. Microbiol. Immunol., (9), 14-21.

The authors declare that there are no conflicts of interest present.