actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.29413/ABS.2019-4.5.9actabiomedica-2170Research ArticleМОРФОЛОГИЯ, ФИЗИОЛОГИЯ И ПАТОФИЗИОЛОГИЯMORPHOLOGY, PHYSIOLOGY AND PATHOPHYSIOLOGYДинамика активности MAP-киназных каскадов в процессе заживления послеоперационной кожно-мышечной раныDynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Woundshttps://orcid.org/0000-0003-3980-050XШурыгинаИ. А.ShuryginaI. A.

доктор медицинских наук, профессор РАН, заместитель директора по науке

664003, г. Иркутск, ул. Борцов Революции, 1, Россия

Dr. Sc. (Med.), Professor of RAS, Deputy Director for Science

Bortsov Revolutsii str. 1, 664003 Irkutsk, Russian Federation

irinashurygina@gmail.comУманецВ. А.UmanetsV. A.

кандидат химических наук, старший научный сотрудник лаборатории клеточных технологий и регенеративной медицины

664003, г. Иркутск, ул. Борцов Революции, 1, Россия

Cand. Sc. (Chem.), Senior Research Officer, Laboratory of Cell Technologies and Regenerative Medicine

Bortsov Revolutsii str. 1, 664003 Irkutsk, Russian Federation

umanets_vitaly@rambler.ruhttps://orcid.org/0000-0001-5921-0318ШурыгинМ. Г.ShuryginM. G.

доктор медицинских наук, заведующий научно-лабораторным отделом, директор по науке и инновационной деятельности

664007, г. Иркутск, ул. Красногвардейская, 3, Россия

Dr. Sc. (Med.), Head of the Scientific Laboratory Department; Director for Science and Innovations

Bortsov Revolutsii str. 1, 664003 Irkutsk, Russian Federation

Krasnogvardeyskaya str. 3, 664007 Irkutsk, Russia Federation

shurygin@rambler.ruФГБНУ «Иркутский научный центр хирургии и травматологии»Irkutsk Scientific Centre of Surgery and TraumatologyАО «Фармасинтез»Irkutsk Scientific Centre of Surgery and Traumatology; “Farmasintez”201912112019455559Copyright © Шурыгина И.А., Уманец В.А., Шурыгин М.Г., 20192019Шурыгина И.А., Уманец В.А., Шурыгин М.Г.Shurygina I.A., Umanets V.A., Shurygin M.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/2170

Обоснование. Управление репаративным процессом – актуальная задача современной медицины. По нашему мнению, перспективна разработка патогенетически обоснованных подходов к оптимизации процесса репарации для управления взаимосоотношением клеточно-стромальных элементов. Одним из перспективных направлений в этом плане является воздействие на МАРК-каскады.Цель. Изучить экспрессию МАР-киназных механизмов в регуляции репарации на примере кожно-мышечной раны.Методы. Линейную кожно-мышечную рану моделировали с использованием крыс линии Wistar весом 220–250 г в возрасте 9 месяцев (n = 24). Проводили иммунофлюоресцентное окрашивание для выявления активности р38-, JNK- и ERK MAPK-каскадов с сроки от 1 до 30 суток.Результаты. Установлено, что специфическое окрашивание в области формирования соединительной ткани при окраске на р38 MAPK и её фосфорилированную форму впервые отмечено на 3-и сутки, и на этот же срок приходилась максимальная её выраженность. На 7-е и 14-е сутки минимально окрашивались небольшие зоны в области формирования рубца. Фосфорилированная часть JNK-каскада в зоне травматического повреждения выявлялась, начиная с 1-х суток после травмы. Яркая окраска сохранялась на 3-и сутки. На 7-е сутки окраска была минимальной, а к 14-м суткам наблюдалась вторая волна экспрессии. Окрашивание на ERK отмечалось с 1-х и до 14-х суток с пиком активности на 3-и суткиЗаключение. Таким образом, нами выявлено одновременное вовлечение в регуляцию репаративного процесса в условиях кожно-мышечной раны р38-, JNK- и ERK-каскадов. При этом обращает на себя внимание, что пиковая активность всех каскадов совпадает и приходится на 3-и сутки.

Background. Management of the reparative process is an urgent task of modern medicine. In our opinion, the development of pathogenetically grounded approaches to optimizing the repair process for managing the interrelations of stromal cells is promising. One of the promising areas in this regard is the impact on the MAPK-cascades.Aim: to study the expression of MAP-kinase mechanisms in the regulation of repair by the example of a musculocutaneous wound.Methods. A linear muscular skin wound was modeled using Wistar rats weighing 220–250 g at the age of 9 months (n = 24). Immunofluorescence staining was performed to detect the activity of p38, JNK, and ERK MAPK cascades from 1 to 30 days.Results. It was established that specific staining in the area of connective tissue formation during staining with p38 MAPK and its phosphorylated form was first observed on the 3rd day, and its maximum severity occurred at the same time. On the 7th and 14th day, small zones in the area of scar formation were minimally stained. The phosphorylated part of the JNK-cascade in the zone of traumatic injury was detected starting from the 1st day after the injury. Bright color persisted on the 3rd day. On the 7th day, the color was minimal, and by the 14th day a second wave of expression was observed. ERK-staining was observed from the 1st to the 14th day with a peak activity on the 3rd day.Conclusion. Thus, we revealed the simultaneous involvement of p38, JNK-, and ERK-cascades in the regulation of the reparative process in the conditions of a musculoskeletal wound. At the same time, it is noteworthy that the peak activity of all cascades coincides and falls on the 3rd day.

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The authors declare that there are no conflicts of interest present.