actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.29413/ABS.2019-4.2.15actabiomedica-2053Research ArticleФАРМАКОЛОГИЯ И ФАРМАЦИЯPHARMACOLOGY AND PHARMACEUTICSИзучение нейропротективного действия экстракта чистотела большого (Chelidonium majus L.) in vitroThe Study of the Neuroprotective Effect of the Extract from Chelidonium Majus L. in VitroЖалсрайА.ZhalsraiA.

Жалсрай Алдармаа – кандидат биологических наук, старший научный сотрудник

210646, г. Улан-Батор, ул. Чингисхана, 214, Монголия

Zhalsrai Aldarmaa – Cand. Sc. (Biol.), Senior Research Officer

ul. Genghis Khan 214, Ulaanbaatar 210646, Mongolia

jaldarmaa@gmail.comСанжиеваЛ. Ц.SanzhievaL. Ts.

Санжиева Людмила Цымпиловна – кандидат биологических наук, доцент кафедры зоологии и экологии

670000, Республика Бурятия, г. Улан-Удэ, ул. Смолина, 24а

Lyudmila Ts. Sanzhieva – Cand. Sc. (Biol.), Associate Professor at the Department of Zoology and Ecology

ul. Smolina 24a, Ulan-Ude 670000

ltssanzh@gmail.comИнститут традиционной медицины и технологииInstitute of Traditional Medicine and TechnologyФГБОУ ВО «Бурятский государственный университет им. Доржи Банзарова»Banzarov Buryat State University20192405201942106113Copyright © Жалсрай А., Санжиева Л.Ц., 20192019Жалсрай А., Санжиева Л.Ц.Zhalsrai A., Sanzhieva L.T.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/2053

Целью данного исследования было изучение нейропротекторного действия экстракта чистотела большого (Chelidonium majus L.). Количественное определение суммарного алкалоида из экстракта Чистотела большого проводили методом титрования. Экстракт чистотела большого продемонстрировал значительное ингибирование свободных липидных радикалов в ESR-анализе, но не уменьшал образование гидроксильных радикалов. Концентрация полумаксимального ингибирования (IC50) свободных липидных радикалов экстрактом составила 2,96 мг/мл, тогда как для гидроксильных радикалов она составила 55,13 мг/мл. Также оценено защитное действие экстракта чистотела большого против митохондриальной дисфункции, вызванной 100 мкМ малондиальдегида, в изолированных митохондриях клеток головного мозга. Экстракт чистотела большого в концентрациях 12 и 25 мг/мл достоверно увеличивал пониженный уровень комплекса I, что указывает на проявление его антиоксидантного действия. Но при этом экстракт не влиял на пониженный уровень активности энзимов электронтранспортной цепи митохондрий (комплекс II и малатдегидрогеназы МДГ), вызванный 100 мкМ малондиальдегида. Также мы изучили защитное действие экстракта против нейротоксичности, вызванной перекисью водорода (H2O2) в культивированных кортикальных нейронах. Показано, что экстракт чистотела большого в концентрациях от 0,05 до 0,5 мг/мл достоверно снижает гибель нейронов, вызванную токсическим действием перекиси водорода (100 мкМ, H2O2).

При определении механизма действия использовался люциферазный тест ARE-luc. Было показано, что экстракт активирует ARE-зависимую экспрессию генов, но не влияет на пониженный уровень NF-E2-зависимого фактора 2 (Nrf2).

Можно предположить, что экстракт чистотела большого оказывает нейрозащитное действие, активируя ARE-зависимый сигнальный путь.

The protection of neurons from damage and death is an important challenge in the development of treatment of brain ischemia and neurodegenerative diseases. This study aims to investigate protective effect of the extract prepared from Chelidonium majus, which contains total alkaloids. In the present study, we examined antioxidant activity of total alkaloids from Chelidonium majus in vitro. Hydroxyl radical and lipid radicals were detected using spin trapping agents with ESR spectrometer. Chelidonium majus extract exhibited dose-dependent scavenging effects on lipid radicals. Halfmaximal inhibitory concentration (IC50) of the extract was 2.96 mg/ml, whereas for hydroxyl radicals it was 55.13 mg/ ml. These results showed that extract of Chelidonium majus is partly inhibited free radicals. Antioxidant effects of this extract were further demonstrated by protecting enzyme activity of the mitochondrial respiratory electron transport chain (complex I) in isolated brain mitochondrial dysfunction induced by MDA. However, it did not change the decreased level of complex II, and malate dehydrogenase (MDH) in a concentration of 12 and 25 mg/ml. Here, we examined the neuroprotective effect of the extract from Chelidonium majus against oxidative stress in cultured cortical neurons, using MTT. We found that pretreatment with the extract of Chelidonium majus (0.05 and 0.5 mg/ml) significantly inhibited H2O2-induced cell death in cortical neurons.

Furthermore, the use of a luciferase reporter (ARE-luc) assay showed that extract from Chelidonium majus activates protective signaling pathway in primary cortical neurons through ARE/Nrf2 pathway.

Together, this suggests that total alkaloids from Chelidonium majus may be neuroprotective by increasing anti-oxidant gene expression.

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The authors declare that there are no conflicts of interest present.