Методом ВЭЖХ проведено исследование фенольных соединений E. pectinata, произрастающей в Иркутской области. Были изучены орган-специфическое распределение и динамика накопления в зависимости от фазы вегетации для 8 соединений. Максимальное количество гликозидов флавоноидов накапливается в цветках и листьях, а агликонов флавоноидов - в стеблях. Максимальное количество флавоноидов в надземных органах наблюдается в период цветения, а фенилэтаноида актеозида - в период плодоношения.
A plant of the Euphrasia L. genus is promising to be introduced into medical practice and being used in traditional medicine for eye, gastrointestinal tract, upper respiratory tract disease treatment. Euphrasia pectinata Ten is widespread and growing abundantly in the Central Siberia. Study of the composition and quantitative content of phenolic compounds of Euphrasia pectinata Ten. growing in Irkutsk Region was studied by HpLC method. Organ-specific distribution and dynamics of accumulation depending on the phase of vegetation of 8 compounds (3-O-caffeoylquinic acid(chlorogenicacid), hyperozide, acteoside, cynaroside, diosmetin-7-O-β-D-glucuronide, dihydroquercetin, luteolin, quercetin) were studied. The maximum of flavonoid glycosides is accumulated in flowers and leaves, and the maximum of flavonoid aglycone is accumulated in stems. The maximum quantity of flavonoids in aboveground organs is observed in the flowering period, and maximum of phenylethanoid acteoside is observed in the fruiting period. Thus, as a medicinal plant, the materials can be recommended for harvesting of the aboveground organs E. pectinata during the flowering period.
The authors declare that there are no conflicts of interest present.