actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problemsactabiomedica-123Research ArticleГЕНЕТИКА И ПРОТЕОМИКАGENETICS AND PROTEOMICSАНАЛИЗ КОМПЛЕКСНОГО НОСИТЕЛЬСТВА ГЕНЕТИЧЕСКИХ ПОЛИМОРФНЫХ ВАРИАНТОВ, АССОЦИИРОВАННЫХ С РАЗЛИЧНЫМИ ТИПАМИ ИШЕМИЧЕСКОГО ИНСУЛЬТАANALYSIS OF COMPLEX CARRIER STATE OF GENETIC POLYMORPHISMS ASSOCIATED WITH VARIOUS ISCHEMIC STROKE TYPESСтрамбовскаяН. Н.StrambovskayaN. N.strambovskaya@yandex.ruГБОУ ВПО «Читинская государственная медицинская академия» Минздрава РоссииResearch Institute of Molecular Medicine for Chita State Medical Academy201528092015059499Copyright © Страмбовская Н.Н., 20152015Страмбовская Н.Н.Strambovskaya N.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/123

Проведен анализ частот генотипов и аллелей26 полиморфных вариантов23 геновуЗЗО больных ишемическим инсультом и 200 здоровых исследуемых соответствующего пола и возраста (р> 0,05). Выявлены ассоциации с заболеваниему SELE-128Ser>Pro, SELP-1087G>A, ApoC3-3238C>G полиморфных маркеров (0Rmm= 1,6; ORmax=3,92). При использовании метода MDR определены предиктивные и протективные для развития лакунарного или атеротромботического ишемического инсульта 2-6-компонентные комбинации генотипов.

Frequencies of genotypes and alleles for 26 polymorphic variants of23 genes were statistically analyzed in 330patients with Ischemic stroke and 200 healthy subjects, matched for age and gender (p > 0,05) after genetic analysis (PCR-Ehf andPCR-Rt). SELP-1087A (OR = 1,6), SELP-1087GA (OR = l,96),SELE-128Pro (OR=2,66),SELE-128SerPro (OR=3,92), ApoC3-3238G (OR = 1,63), ApoC3-3238CG (OR = 1,95) alleles and genotypes were revealed to be associated with the disease independently (p < 0,05). MDR-method showed 2-6 components combinations of genotypes associated with the (non) development of lacunar ischemic stroke (prediction complexes: SELE-128Ser/ProxNOS3-786C/C (OR = 1,6) and PAI-1675/5G/4GxNOS3-786T/T*NOSl-84G/AxVEGF-A-2578A/C (OR = 6,0); protection complex: SELE-128Ser/ SerxNOS3-786C/T, OR=0,09) and atherothrombotic ischemic stroke (prediction complexes: NOSl-84A/AxApoC3- 3238C/C (OR = 3,0) and SELE-128Ser/ProxFRb-455G/AxNOSl-84G/A (OR = 10,0); protection complex: NOS1-84G/ GxApoC3-3238C/C (OR = 0,31)).

лакунарный ишемический инсультатеротромботический ишемический инсультгенетический полиморфизммультигенное наследованиеlacunar ischemic strokeatherothrombotic ischemic strokegenetic polymorphismmultigenic inheritanceReferencesГенетический паспорт - основа индивидуальной и предиктивной медицины / Под ред. В.С. Баранова. - СПб.: Изд-во Н-Л. - 2009. - 528 с.Генетический паспорт - основа индивидуальной и предиктивной медицины / Под ред. В.С. Баранова. - СПб.: Изд-во Н-Л. - 2009. - 528 с.Суслина ЗА, Пирадов МА Инсульт: диагностика, лечение, профилактика. - М.: МЕДпресс-информ, 2008.-288 с.Суслина ЗА, Пирадов МА Инсульт: диагностика, лечение, профилактика. - М.: МЕДпресс-информ, 2008.-288 с.Cheng YC, Anderson CD, Bione S, Keene K, Maguire JM, Nalls M, Rasheed A, Zeginigg M, Attia J, Baker R, Barlera S, Biffi A, Bookman E, Brott TG, Brown RD Jr., Chen F, Chen WM, Ciusani E, Cole JW, Cortellini L, Danesh J, Doheny K, Ferrucci L, Grazia Franzosi M, Frossard P, Fu- rie KL, Golledge J, Hankey GJ, Hernandez D, Holliday EG, Hsu FC, Jannes J, Kamal A, Khan MS, Kittner SJ, Koblar SA, Lewis M, Lincz L, Lisa A, Matarin M, Moscato P, Mychal- eckyj JC, Parati EA, Parolo S, Pugh E, Rost NS, Schallert M, Schmidt H, Scott RJ, Sturm JW, Yadav S, Zaidi M, Boncora- glio GB, Levi CR, Meschia JF, Rosand J, Sale M, Saleheen D, Schmidt R, Sharma P, Worrall B, Mitchell BD; GARNET Collaborative Research Group; GENEVA Consortium; International Stroke Genetics Consortium. Are myocardial infarction-associated single-nucleotide polymorphisms associated with ischemic stroke? Stroke. 2012, 43 (4), 980-986.Cheng YC, Anderson CD, Bione S, Keene K, Maguire JM, Nalls M, Rasheed A, Zeginigg M, Attia J, Baker R, Barlera S, Biffi A, Bookman E, Brott TG, Brown RD Jr., Chen F, Chen WM, Ciusani E, Cole JW, Cortellini L, Danesh J, Doheny K, Ferrucci L, Grazia Franzosi M, Frossard P, Fu- rie KL, Golledge J, Hankey GJ, Hernandez D, Holliday EG, Hsu FC, Jannes J, Kamal A, Khan MS, Kittner SJ, Koblar SA, Lewis M, Lincz L, Lisa A, Matarin M, Moscato P, Mychal- eckyj JC, Parati EA, Parolo S, Pugh E, Rost NS, Schallert M, Schmidt H, Scott RJ, Sturm JW, Yadav S, Zaidi M, Boncora- glio GB, Levi CR, Meschia JF, Rosand J, Sale M, Saleheen D, Schmidt R, Sharma P, Worrall B, Mitchell BD; GARNET Collaborative Research Group; GENEVA Consortium; International Stroke Genetics Consortium. Are myocardial infarction-associated single-nucleotide polymorphisms associated with ischemic stroke? Stroke. 2012, 43 (4), 980-986.Debette S, Seshadri S. (2009). Genetics of athero- thrombotic and lacunar stroke. Circ. Cardiovasc Genet., 1,2(2), 191-198.Debette S, Seshadri S. (2009). Genetics of athero- thrombotic and lacunar stroke. Circ. Cardiovasc Genet., 1,2(2), 191-198.Dichgans M, Hegele RA (2009). Advances in stroke 2008: Update on the genetics of stroke and cerebrovascular disease 2008. Stroke, 40 (5), 289-291.Dichgans M, Hegele RA (2009). Advances in stroke 2008: Update on the genetics of stroke and cerebrovascular disease 2008. Stroke, 40 (5), 289-291.Kubo M, Hata J, Ninomiya T, Matsuda K, Yonemo- to K, Nakano T, Matsushita T, Yamazaki K, Ohnishi Y, Saito S, Kitazono T, Ibayashi S, Sueishi K, Iida M, Nakamura Y, Kiy- oharaY (2007). AnonsynonymousSNPin PRKCH (protein kinase C eta) increases the risk of cerebral infarction. Nat Genet., 39 (2), 212-217.Kubo M, Hata J, Ninomiya T, Matsuda K, Yonemo- to K, Nakano T, Matsushita T, Yamazaki K, Ohnishi Y, Saito S, Kitazono T, Ibayashi S, Sueishi K, Iida M, Nakamura Y, Kiy- oharaY (2007). AnonsynonymousSNPin PRKCH (protein kinase C eta) increases the risk of cerebral infarction. Nat Genet., 39 (2), 212-217.Lanktree MB, Dichgans M, Hegele RA (2010). Advances in genomic analysis of stroke. What have we learned and where are we headed. Stroke, 41 (4), 825-832.Lanktree MB, Dichgans M, Hegele RA (2010). Advances in genomic analysis of stroke. What have we learned and where are we headed. Stroke, 41 (4), 825-832.Matarin M, Brown WM, Dena H, Britton A, De Vrieze FW, BrottTG, Brown RD, Worrall BB, Case LD, Ch- anock SJ, Metter EJ, Ferruci L, Gamble D, Hardy JA, Rich SS, Singleton A, Meschia JF (2009). Candidate gene polymorphisms for ischemic stroke. Stroke, 40 (11), 3436-3442.Matarin M, Brown WM, Dena H, Britton A, De Vrieze FW, BrottTG, Brown RD, Worrall BB, Case LD, Ch- anock SJ, Metter EJ, Ferruci L, Gamble D, Hardy JA, Rich SS, Singleton A, Meschia JF (2009). Candidate gene polymorphisms for ischemic stroke. Stroke, 40 (11), 3436-3442.Meschia JF (2011). Advances in genetics 2010. Stroke, 42 (2), 285-287.Meschia JF (2011). Advances in genetics 2010. Stroke, 42 (2), 285-287.MeschiaJF,Tournier-LasserveE (2013). Advances in stroke. Genetics 2012. Stroke, 44 (2), 309-310.MeschiaJF,Tournier-LasserveE (2013). Advances in stroke. Genetics 2012. Stroke, 44 (2), 309-310.Nadifi S, Hamzi К (2012). Stroke, epidemiological and genetical approach. Neurodegeneration, 12, 279-302.Nadifi S, Hamzi К (2012). Stroke, epidemiological and genetical approach. Neurodegeneration, 12, 279-302.Pezzini A, Grassi M, Del Zotto E, Archetti S, SpeziR,VerganiV,Assanelli D,Caimi L, Padovani A(2005). Cumulative effect of predisposing genotypes and their interaction with modifiable factors on the risk of ischemic stroke in young adults. Stroke, 36 (3), 533-539Pezzini A, Grassi M, Del Zotto E, Archetti S, SpeziR,VerganiV,Assanelli D,Caimi L, Padovani A(2005). Cumulative effect of predisposing genotypes and their interaction with modifiable factors on the risk of ischemic stroke in young adults. Stroke, 36 (3), 533-539

The authors declare that there are no conflicts of interest present.